A novel reporter system for neutralizing and enhancing antibody assay against dengue virus
Identifieur interne : 001191 ( Main/Exploration ); précédent : 001190; suivant : 001192A novel reporter system for neutralizing and enhancing antibody assay against dengue virus
Auteurs : Ke-Yu Song [République populaire de Chine] ; Hui Zhao [République populaire de Chine] ; Zhen-You Jiang [République populaire de Chine] ; Xiao-Feng Li [République populaire de Chine] ; Yong-Qiang Deng [République populaire de Chine] ; Tao Jiang [République populaire de Chine] ; Shun-Ya Zhu [République populaire de Chine] ; Pei-Yong Shi [Singapour] ; Bo Zhang [République populaire de Chine] ; Fu-Chun Zhang [République populaire de Chine] ; E-De Qin [République populaire de Chine] ; Cheng-Feng Qin [République populaire de Chine]Source :
- BMC Microbiology [ 1471-2180 ] ; 2014.
Abstract
Dengue virus (DENV) still poses a global public health threat, and no vaccine or antiviral therapy is currently available. Antibody plays distinct roles in controlling DENV infections. Neutralizing antibody is protective against DENV infection, whereas sub-neutralizing concentration of antibody can increase DENV infection, termed antibody-dependent enhancement (ADE). Plaque-based assay represents the most widely accepted method measuring neutralizing or enhancing antibodies.
In this study, a novel reporter virus-based system was developed for measuring neutralization and ADE activity. A stable
This reporter virus-based assay for neutralizing and enhancing antibody evaluation is rapid, lower cost, and high throughput, and will be helpful for laboratory detection and epidemiological investigation for DENV antibodies.
The online version of this article (doi:10.1186/1471-2180-14-44) contains supplementary material, which is available to authorized users.
Url:
DOI: 10.1186/1471-2180-14-44
PubMed: 24548533
PubMed Central: 3930823
Affiliations:
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id="Aff2"><institution-wrap><institution-id institution-id-type="GRID">grid.258164.c</institution-id><institution-id institution-id-type="ISNI">0000000417903548</institution-id><institution>Department of Microbiology and Immunology,</institution><institution>School of Medicine Jinan University,</institution></institution-wrap>Guangzhou, 510632 China</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Guangzhou</wicri:regionArea></affiliation></author><author><name sortKey="Zhao, Hui" sort="Zhao, Hui" uniqKey="Zhao H" first="Hui" last="Zhao">Hui Zhao</name><affiliation wicri:level="1"><nlm:aff id="Aff1"><institution-wrap><institution-id institution-id-type="GRID">grid.410576.1</institution-id><institution>Department of Virology, State Key Laboratory of Pathogen and Biosecurity,</institution><institution>Beijing Institute of Microbiology and Epidemiology,</institution></institution-wrap>Beijing, 100071 China</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Beijing</wicri:regionArea></affiliation></author><author><name sortKey="Jiang, Zhen You" sort="Jiang, Zhen You" uniqKey="Jiang Z" first="Zhen-You" last="Jiang">Zhen-You Jiang</name><affiliation wicri:level="1"><nlm:aff id="Aff2"><institution-wrap><institution-id institution-id-type="GRID">grid.258164.c</institution-id><institution-id institution-id-type="ISNI">0000000417903548</institution-id><institution>Department of Microbiology and Immunology,</institution><institution>School of Medicine Jinan University,</institution></institution-wrap>Guangzhou, 510632 China</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Guangzhou</wicri:regionArea></affiliation></author><author><name sortKey="Li, Xiao Feng" sort="Li, Xiao Feng" uniqKey="Li X" first="Xiao-Feng" last="Li">Xiao-Feng Li</name><affiliation wicri:level="1"><nlm:aff id="Aff1"><institution-wrap><institution-id institution-id-type="GRID">grid.410576.1</institution-id><institution>Department of Virology, State Key Laboratory of Pathogen and Biosecurity,</institution><institution>Beijing Institute of Microbiology and Epidemiology,</institution></institution-wrap>Beijing, 100071 China</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Beijing</wicri:regionArea></affiliation></author><author><name sortKey="Deng, Yong Qiang" sort="Deng, Yong Qiang" uniqKey="Deng Y" first="Yong-Qiang" last="Deng">Yong-Qiang Deng</name><affiliation wicri:level="1"><nlm:aff id="Aff1"><institution-wrap><institution-id institution-id-type="GRID">grid.410576.1</institution-id><institution>Department of Virology, State Key Laboratory of Pathogen and Biosecurity,</institution><institution>Beijing Institute of Microbiology and Epidemiology,</institution></institution-wrap>Beijing, 100071 China</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Beijing</wicri:regionArea></affiliation></author><author><name sortKey="Jiang, Tao" sort="Jiang, Tao" uniqKey="Jiang T" first="Tao" last="Jiang">Tao Jiang</name><affiliation wicri:level="1"><nlm:aff id="Aff1"><institution-wrap><institution-id institution-id-type="GRID">grid.410576.1</institution-id><institution>Department of Virology, State Key Laboratory of Pathogen and Biosecurity,</institution><institution>Beijing Institute of Microbiology and Epidemiology,</institution></institution-wrap>Beijing, 100071 China</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Beijing</wicri:regionArea></affiliation></author><author><name sortKey="Zhu, Shun Ya" sort="Zhu, Shun Ya" uniqKey="Zhu S" first="Shun-Ya" last="Zhu">Shun-Ya Zhu</name><affiliation wicri:level="1"><nlm:aff id="Aff1"><institution-wrap><institution-id institution-id-type="GRID">grid.410576.1</institution-id><institution>Department of Virology, State Key Laboratory of Pathogen and Biosecurity,</institution><institution>Beijing Institute of Microbiology and Epidemiology,</institution></institution-wrap>Beijing, 100071 China</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Beijing</wicri:regionArea></affiliation></author><author><name sortKey="Shi, Pei Yong" sort="Shi, Pei Yong" uniqKey="Shi P" first="Pei-Yong" last="Shi">Pei-Yong Shi</name><affiliation wicri:level="1"><nlm:aff id="Aff3"><institution-wrap><institution-id institution-id-type="GRID">grid.410761.5</institution-id><institution>Novartis Institute for Tropical Diseases,</institution></institution-wrap>Singapore, 138670 Singapore</nlm:aff><country xml:lang="fr">Singapour</country><wicri:regionArea>Singapore</wicri:regionArea></affiliation></author><author><name sortKey="Zhang, Bo" sort="Zhang, Bo" uniqKey="Zhang B" first="Bo" last="Zhang">Bo Zhang</name><affiliation wicri:level="1"><nlm:aff id="Aff4"><institution-wrap><institution-id institution-id-type="GRID">grid.439104.b</institution-id><institution-id institution-id-type="ISNI">0000000417981925</institution-id><institution>State Key Laboratory of Virology,</institution><institution>Wuhan Institute of Virology CAS,</institution></institution-wrap>Wuhan, 430072 China</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Wuhan</wicri:regionArea></affiliation></author><author><name sortKey="Zhang, Fu Chun" sort="Zhang, Fu Chun" uniqKey="Zhang F" first="Fu-Chun" last="Zhang">Fu-Chun Zhang</name><affiliation wicri:level="3"><nlm:aff id="Aff5">Guangzhou No.8 People’s Hospital, Guangzhou, 510060 China</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Guangzhou No.8 People’s Hospital, Guangzhou</wicri:regionArea><placeName><settlement type="city">Jiangmen</settlement><region type="province">Guangdong</region></placeName></affiliation></author><author><name sortKey="Qin, E De" sort="Qin, E De" uniqKey="Qin E" first="E-De" last="Qin">E-De Qin</name><affiliation wicri:level="1"><nlm:aff id="Aff1"><institution-wrap><institution-id institution-id-type="GRID">grid.410576.1</institution-id><institution>Department of Virology, State Key Laboratory of Pathogen and Biosecurity,</institution><institution>Beijing Institute of Microbiology and Epidemiology,</institution></institution-wrap>Beijing, 100071 China</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Beijing</wicri:regionArea></affiliation></author><author><name sortKey="Qin, Cheng Feng" sort="Qin, Cheng Feng" uniqKey="Qin C" first="Cheng-Feng" last="Qin">Cheng-Feng Qin</name><affiliation wicri:level="1"><nlm:aff id="Aff1"><institution-wrap><institution-id institution-id-type="GRID">grid.410576.1</institution-id><institution>Department of Virology, State Key Laboratory of Pathogen and Biosecurity,</institution><institution>Beijing Institute of Microbiology and Epidemiology,</institution></institution-wrap>Beijing, 100071 China</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Beijing</wicri:regionArea></affiliation></author></analytic><series><title level="j">BMC Microbiology</title><idno type="eISSN">1471-2180</idno><imprint><date when="2014">2014</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><sec><title>Background</title><p>Dengue virus (DENV) still poses a global public health threat, and no vaccine or antiviral therapy is currently available. Antibody plays distinct roles in controlling DENV infections. Neutralizing antibody is protective against DENV infection, whereas sub-neutralizing concentration of antibody can increase DENV infection, termed antibody-dependent enhancement (ADE). Plaque-based assay represents the most widely accepted method measuring neutralizing or enhancing antibodies.</p></sec><sec><title>Results</title><p>In this study, a novel reporter virus-based system was developed for measuring neutralization and ADE activity. A stable <italic>Renilla</italic> luciferase reporter DENV (Luc-DENV) that can produce robust luciferase signals in BHK-21 and K562 cells were used to establish the assay and validated against traditional plaque-based assay. Luciferase value analysis using various known DENV-specific monoclonal antibodies showed good repeatability and a well linear correlation with conventional plaque-based assays. The newly developed assay was finally validated with clinical samples from infected animals and individuals.</p></sec><sec><title>Conclusions</title><p>This reporter virus-based assay for neutralizing and enhancing antibody evaluation is rapid, lower cost, and high throughput, and will be helpful for laboratory detection and epidemiological investigation for DENV antibodies.</p></sec><sec><title>Electronic supplementary material</title><p>The online version of this article (doi:10.1186/1471-2180-14-44) contains supplementary material, which is available to authorized users.</p></sec></div></front><back><div1 type="bibliography"><listBibl><biblStruct><analytic><author><name sortKey="Gubler, Dj" uniqKey="Gubler D">DJ Gubler</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Simmons, Cp" uniqKey="Simmons C">CP Simmons</name></author><author><name sortKey="Farrar, Jj" uniqKey="Farrar J">JJ Farrar</name></author><author><name sortKey="Nguyen, Vv" 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populaire de Chine"><noRegion><name sortKey="Song, Ke Yu" sort="Song, Ke Yu" uniqKey="Song K" first="Ke-Yu" last="Song">Ke-Yu Song</name></noRegion><name sortKey="Deng, Yong Qiang" sort="Deng, Yong Qiang" uniqKey="Deng Y" first="Yong-Qiang" last="Deng">Yong-Qiang Deng</name><name sortKey="Jiang, Tao" sort="Jiang, Tao" uniqKey="Jiang T" first="Tao" last="Jiang">Tao Jiang</name><name sortKey="Jiang, Zhen You" sort="Jiang, Zhen You" uniqKey="Jiang Z" first="Zhen-You" last="Jiang">Zhen-You Jiang</name><name sortKey="Li, Xiao Feng" sort="Li, Xiao Feng" uniqKey="Li X" first="Xiao-Feng" last="Li">Xiao-Feng Li</name><name sortKey="Qin, Cheng Feng" sort="Qin, Cheng Feng" uniqKey="Qin C" first="Cheng-Feng" last="Qin">Cheng-Feng Qin</name><name sortKey="Qin, E De" sort="Qin, E De" uniqKey="Qin E" first="E-De" last="Qin">E-De Qin</name><name sortKey="Song, Ke Yu" sort="Song, Ke Yu" uniqKey="Song K" first="Ke-Yu" last="Song">Ke-Yu Song</name><name sortKey="Zhang, Bo" sort="Zhang, Bo" uniqKey="Zhang B" first="Bo" last="Zhang">Bo Zhang</name><name sortKey="Zhang, Fu Chun" sort="Zhang, Fu Chun" uniqKey="Zhang F" first="Fu-Chun" last="Zhang">Fu-Chun Zhang</name><name sortKey="Zhao, Hui" sort="Zhao, Hui" uniqKey="Zhao H" first="Hui" last="Zhao">Hui Zhao</name><name sortKey="Zhu, Shun Ya" sort="Zhu, Shun Ya" uniqKey="Zhu S" first="Shun-Ya" last="Zhu">Shun-Ya Zhu</name></country><country name="Singapour"><noRegion><name sortKey="Shi, Pei Yong" sort="Shi, Pei Yong" uniqKey="Shi P" first="Pei-Yong" last="Shi">Pei-Yong Shi</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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